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  • Molecular characterization and functional analysis of tyrosine hydroxylase gene in Helicoverpa armigera (Lepidoptera: Noctuidae).

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    Author(s) : Yao XueLi LinHongWei JiZhenXi YuQiangDu MengFangCui JiangKuanAn ShiHengLiu XiaoGuang

    Author Affiliation : State Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China.

    Author Email : yaoxue983@163.comxgliu2000@aliyun.com

    Journal article : Acta Entomologica Sinica 2019 Vol.62 No.3 pp.294-303 ref.24

    Abstract : Aim: Tyrosine hydroxylase (TH) is a key enzyme of melanin biosynthesis and plays an important role in epidermal sclerotization. This study aims to obtain the cDNA sequence of TH gene of Helicoverpa armigerahelicoverpa armigeraSubject Category: Organism Names
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    , and to analyze its molecular characteristics, expression pattern and function, so as to provide a foundation for further exploring its action mechanism. Methods: The full-length cDNA sequence of TH gene was cloned from H. armigera by using bioinformaticsbioinformaticsSubject Category: Disciplines, Occupations and Industries
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    and molecular cloning technique. The developmental expression pattern of this gene was assayed with qRT-PCR. The expression of TH gene in the 5th instar larva of H. armigera after treatment with 20 E (20-hydroxyecdysone) (400 ng/individual) for different time and RNAi of ecdysone receptor gene (EcR) followed by 20E treatment (400 ng/individual) was analyzed by qRT-PCR, respectively. The TH acitivity in the fat bodyfat bodySubject Category: Anatomical and Morphological Structures
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    of H. armigera larvae after treatment with bio-stimulants bursicon (30 µg/mg tissue) and cAMP (200 ng/mg tissue) was determined by biochemical method. Results: We cloned the full-length cDNA sequence of TH (GenBank accession no.: MF440319) from H. armigera, which is 2 270 bp in length with a 1 686 bp open reading frame encoding 561 amino acids. TH is ubiquitously expressed in all the examined developmental stagesdevelopmental stagesSubject Category: Miscellaneous
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    of H. armigera and relatively highly expressed in day-3 of egg stage, day-1 of the 2nd instar larval stage, 3rd-5th instar molting stages, prepupal stage and 1 day-old adult. In vitro treatment, the steroid hormone 20E (400 ng/individual) significantly up-regulated the transcriptiontranscriptionSubject Category: Natural Processes
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    level of TH, but the 20E treatment after RNAi-mediated knockdown of 20E receptor gene EcR showed no obvious effect on the transcription of TH as compared with the control (injected with dsGFP only). Enzymatic activity assay demonstrated that both bursicon (30 µg/mg tissue) and cAMP (200 ng/mg tissue) led to significant increase of the TH activity. Conclusion: 20E is involved in the regulation of TH expression at the transcriptional level, and both bursicon and cAMP can promote the TH activity at the protein level.

    ISSN : 0454-6296

    URL : http://www.insect.org.cn/EN/volumn/cu...

    Record Number : 20193190971

    Publisher : Editorial Office of Acta Entomologica Sinica

    Location of publication : Beijing

    Country of publication : China

    Language of text : Chinese

    Language of summary : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Helicoverpa armigerahelicoverpa armigeraSubject Category: Organism Names
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    , insectsinsectsSubject Category: Organism Names
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    Descriptor(s) : arthropod pestsarthropod pestsSubject Category: Organism Groups
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    , bioinformaticsbioinformaticsSubject Category: Disciplines, Occupations and Industries
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    , c-AMPc-ampSubject Category: Chemicals and Chemical Groups
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    , complementary DNAcomplementary dnaSubject Category: Chemicals and Chemical Groups
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    , developmental stagesdevelopmental stagesSubject Category: Miscellaneous
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    , ecdysteroneecdysteroneSubject Category: Chemicals and Chemical Groups
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    , enzyme activityenzyme activitySubject Category: Properties
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    , enzymesenzymesSubject Category: Chemicals and Chemical Groups
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    , fat bodyfat bodySubject Category: Anatomical and Morphological Structures
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    , gene expressiongene expressionSubject Category: Miscellaneous
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    , genesgenesSubject Category: Miscellaneous
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    , insect pestsinsect pestsSubject Category: Organism Groups
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    , messenger RNAmessenger rnaSubject Category: Chemicals and Chemical Groups
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    , nucleotide sequencesnucleotide sequencesSubject Category: Chemicals and Chemical Groups
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    , open reading framesopen reading framesSubject Category: Chemicals and Chemical Groups
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    , pestspestsSubject Category: Organism Groups
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    , plant pestsplant pestsSubject Category: Organism Groups
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    , polymerase chain reactionpolymerase chain reactionSubject Category: Techniques, Methodologies and Equipment
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    , RNA interferencerna interferenceSubject Category: Miscellaneous
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    , transcriptiontranscriptionSubject Category: Natural Processes
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    , tyrosine 3-monooxygenasetyrosine 3-monooxygenaseSubject Category: Chemicals and Chemical Groups
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    Identifier(s) : African cotton bollworm, cDNA, cyclic adenosine monophosphate, cyclic AMP, DNA sequences, DNA transcription, growth phase, mRNA, ORFs, PCR, pest arthropods, pest insects, RNAi, tyrosine hydroxylase

    Broader term(s) : HelicoverpahelicoverpaSubject Category: Organism Names
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    , NoctuidaenoctuidaeSubject Category: Organism Names
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    , LepidopteralepidopteraSubject Category: Organism Names
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    , insectsinsectsSubject Category: Organism Names
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    , HexapodahexapodaSubject Category: Organism Names
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    , arthropodsarthropodsSubject Category: Organism Names
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    , invertebratesinvertebratesSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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