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Author(s) :
Yao Xue
;
Li LinHong
;
Wei JiZhen
;
Xi YuQiang
;
Du MengFang
;
Cui JiangKuan
;
An ShiHeng
;
Liu XiaoGuang
Author Affiliation :
State Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China.
Author Email :
yaoxue983@163.com
,
xgliu2000@aliyun.com
Journal article
:
Acta Entomologica Sinica
2019
Vol.62
No.3
pp.294-303
ref.24
Abstract :
Aim: Tyrosine hydroxylase (TH) is a key enzyme of melanin biosynthesis and plays an important role in epidermal sclerotization. This study aims to obtain the cDNA sequence of TH gene of
Helicoverpa armigerahelicoverpa armigeraSubject Category: Organism Names
see more details, and to analyze its molecular characteristics, expression pattern and function, so as to provide a foundation for further exploring its action mechanism. Methods: The full-length cDNA sequence of TH gene was cloned from
H. armigera by using
bioinformaticsbioinformaticsSubject Category: Disciplines, Occupations and Industries
see more details and molecular cloning technique. The developmental expression pattern of this gene was assayed with qRT-PCR. The expression of TH gene in the 5th instar larva of
H. armigera after treatment with 20 E (20-hydroxyecdysone) (400 ng/individual) for different time and RNAi of ecdysone receptor gene (
EcR) followed by 20E treatment (400 ng/individual) was analyzed by qRT-PCR, respectively. The TH acitivity in the
fat bodyfat bodySubject Category: Anatomical and Morphological Structures
see more details of
H. armigera larvae after treatment with bio-stimulants bursicon (30 µg/mg tissue) and cAMP (200 ng/mg tissue) was determined by biochemical method. Results: We cloned the full-length cDNA sequence of
TH (GenBank accession no.: MF440319) from
H. armigera, which is 2 270 bp in length with a 1 686 bp open reading frame encoding 561 amino acids.
TH is ubiquitously expressed in all the examined
developmental stagesdevelopmental stagesSubject Category: Miscellaneous
see more details of
H. armigera and relatively highly expressed in day-3 of egg stage, day-1 of the 2nd instar larval stage, 3rd-5th instar molting stages, prepupal stage and 1 day-old adult.
In vitro treatment, the steroid hormone 20E (400 ng/individual) significantly up-regulated the
transcriptiontranscriptionSubject Category: Natural Processes
see more details level of
TH, but the 20E treatment after RNAi-mediated knockdown of 20E receptor gene
EcR showed no obvious effect on the transcription of
TH as compared with the control (injected with ds
GFP only). Enzymatic activity assay demonstrated that both bursicon (30 µg/mg tissue) and cAMP (200 ng/mg tissue) led to significant increase of the TH activity. Conclusion: 20E is involved in the regulation of
TH expression at the transcriptional level, and both bursicon and cAMP can promote the TH activity at the protein level.
ISSN :
0454-6296
URL :
http://www.insect.org.cn/EN/volumn/cu...
Record Number :
20193190971
Publisher :
Editorial Office of Acta Entomologica Sinica
Location of publication :
Beijing
Country of publication :
China
Language of text :
Chinese
Language of summary :
English
Indexing terms for this abstract:
Organism descriptor(s) :
Helicoverpa armigerahelicoverpa armigeraSubject Category: Organism Names
see more details, insectsinsectsSubject Category: Organism Names
see more details
Descriptor(s) :
arthropod pestsarthropod pestsSubject Category: Organism Groups
see more details, bioinformaticsbioinformaticsSubject Category: Disciplines, Occupations and Industries
see more details, c-AMPc-ampSubject Category: Chemicals and Chemical Groups
see more details, complementary DNAcomplementary dnaSubject Category: Chemicals and Chemical Groups
see more details, developmental stagesdevelopmental stagesSubject Category: Miscellaneous
see more details, ecdysteroneecdysteroneSubject Category: Chemicals and Chemical Groups
see more details, enzyme activityenzyme activitySubject Category: Properties
see more details, enzymesenzymesSubject Category: Chemicals and Chemical Groups
see more details, fat bodyfat bodySubject Category: Anatomical and Morphological Structures
see more details, gene expressiongene expressionSubject Category: Miscellaneous
see more details, genesgenesSubject Category: Miscellaneous
see more details, insect pestsinsect pestsSubject Category: Organism Groups
see more details, messenger RNAmessenger rnaSubject Category: Chemicals and Chemical Groups
see more details, nucleotide sequencesnucleotide sequencesSubject Category: Chemicals and Chemical Groups
see more details, open reading framesopen reading framesSubject Category: Chemicals and Chemical Groups
see more details, pestspestsSubject Category: Organism Groups
see more details, plant pestsplant pestsSubject Category: Organism Groups
see more details, polymerase chain reactionpolymerase chain reactionSubject Category: Techniques, Methodologies and Equipment
see more details, RNA interferencerna interferenceSubject Category: Miscellaneous
see more details, transcriptiontranscriptionSubject Category: Natural Processes
see more details, tyrosine 3-monooxygenasetyrosine 3-monooxygenaseSubject Category: Chemicals and Chemical Groups
see more details
Identifier(s) :
African cotton bollworm, cDNA, cyclic adenosine monophosphate, cyclic AMP, DNA sequences, DNA transcription, growth phase, mRNA, ORFs, PCR, pest arthropods, pest insects, RNAi, tyrosine hydroxylase
Broader term(s) :
HelicoverpahelicoverpaSubject Category: Organism Names
see more details, NoctuidaenoctuidaeSubject Category: Organism Names
see more details, LepidopteralepidopteraSubject Category: Organism Names
see more details, insectsinsectsSubject Category: Organism Names
see more details, HexapodahexapodaSubject Category: Organism Names
see more details, arthropodsarthropodsSubject Category: Organism Names
see more details, invertebratesinvertebratesSubject Category: Organism Names
see more details, animalsanimalsSubject Category: Organism Names
see more details, eukaryoteseukaryotesSubject Category: Organism Names
see more details