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  • Discrimination between Asian populations of the parasitoid wasp Ganaspis cf. brasiliensis using a simple MALDI-TOF MS-based method for use with insects.

    Author(s) : Reeve, M. A.Seehausen, M. L.

    Author Affiliation : CABI, Bakeham Lane, Egham, Surrey, TW20 9TY, UK.

    Author Email : M.Reeve@cabi.org

    Journal article : Biology Methods & Protocols 2019 Vol.4 No.1 ref.25

    Abstract : The fruit fly Drosophila suzukii has recently become an invasive pest insect of significant economic impact in Europe and the USA. In contrast to other Drosophila species, D. suzukii is able to infest intact fruit by means of a saw-like ovipositor, which allows females to deposit eggs beneath the skin of the fruit. Classical biological control using the parasitoid wasp GanaspisganaspisSubject Category: Organism Names
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    cf. brasiliensis is currently being researched as an environmentally sustainable option for the control of D. suzukii. In particular, the host specificity of this parasitoid has been assessed for populations from different regions in ChinachinaSubject Category: Geographic Entities
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    and JapanjapanSubject Category: Geographic Entities
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    . In order to study the relationship between the differences in specificity and molecular variations, we have adapted a matrix-assisted laser-desorption and ionization time-of-flight mass spectrometry (MALDI-TOFmaldi-tofSubject Category: Techniques, Methodologies and Equipment
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    MS)-based method, originally developed for use with plant material, to discriminate between example populations of G. cf. brasiliensis. We have employed a combination of principal component analysis and blind-tested comparison between reference sample MALDI-TOF MS spectra and test sample spectra to discriminate, on the basis of the acid-soluble insect protein spectra generated, between four populations of G. cf. brasiliensis (originally collected from Tokyo and Hasuike in Japan and Dali and Ximing in China). MALDI-TOF MS analysis is able to discriminate with 100% accuracy between populations G. cf. brasiliensis. The Chinese populations were observed to be similar, but the Tokyo population is slightly different and the Hasuike population is significantly different from the other populations. The Tokyo population appears more closely related to the Chinese populations than the Hasuike population, even though both originate from Japan.

    ISSN : 2396-8923

    DOI : 10.1093/biomethods/bpz002

    Record Number : 20203347928

    Publisher : Oxford University Press

    Location of publication : Oxford

    Country of publication : UK

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : GanaspisganaspisSubject Category: Organism Names
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    , insectsinsectsSubject Category: Organism Names
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    Descriptor(s) : MALDI-TOFmaldi-tofSubject Category: Techniques, Methodologies and Equipment
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    , plant pestsplant pestsSubject Category: Organism Groups
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    , insect pestsinsect pestsSubject Category: Organism Groups
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    , parasitoidsparasitoidsSubject Category: Organism Groups
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    , natural enemiesnatural enemiesSubject Category: Organism Groups
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    , biological control agentsbiological control agentsSubject Category: Organism Groups
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    , techniquestechniquesSubject Category: Techniques, Methodologies and Equipment
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    , analytical methodsanalytical methodsSubject Category: Techniques, Methodologies and Equipment
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    , proteinsproteinsSubject Category: Chemicals and Chemical Groups
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    , pestspestsSubject Category: Organism Groups
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    , arthropod pestsarthropod pestsSubject Category: Organism Groups
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    , parasitesparasitesSubject Category: Organism Groups
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    Identifier(s) : Ganaspis brasiliensis, matrix-associated laser desorption ionization time of flight mass spectrometry, pest insects, biological control organisms, biocontrol agents, analytical techniques, People's Republic of China, pest arthropods

    Geographical Location(s) : JapanjapanSubject Category: Geographic Entities
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    , ChinachinaSubject Category: Geographic Entities
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    Broader term(s) : APEC countriesapec countriesSubject Category: Institutions and Organisations
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    , Developed Countriesdeveloped countriesSubject Category: Miscellaneous
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    , East Asiaeast asiaSubject Category: Geographic Entities
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    , AsiaasiaSubject Category: Geographic Entities
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    , OECD Countriesoecd countriesSubject Category: Institutions and Organisations
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    , FigitidaefigitidaeSubject Category: Organism Names
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    , HymenopterahymenopteraSubject Category: Organism Names
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    , insectsinsectsSubject Category: Organism Names
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    , HexapodahexapodaSubject Category: Organism Names
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    , arthropodsarthropodsSubject Category: Organism Names
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    , invertebratesinvertebratesSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , Developing Countriesdeveloping countriesSubject Category: Miscellaneous
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